不同饲养方式对乌鳢(Ophiocephalus argus)肠道微生物群落结构差异及种类多样性的影响     

盛鹏程  周冬仁  韩新荣  胡晓波  叶雪平  徐磊  周聃  吴琦芳  郝贵杰 《海洋与湖沼》2020,51(1):148-155

以乌鳢(Ophiocephalus argus)为研究对象,采用不同日粮饲喂,试验W1组饲喂冰鲜鱼,试验W2组饲喂畜禽内脏。12周后,取肠道内容物,利用16S rDNA技术研究投喂不同饲粮的乌鳢肠道生物群落结构差异和微生物多样性,分别获得W1组和W2组乌鳢肠道细菌有效序列51124和39298条。结果分析表明;W1组和W2组物种注释(operational taxonomic unit,OTU)数目分别为2631和4114条,分类地位明确的细菌种类分别隶属5个属和49个属,W1组和W2组乌鳢肠道细菌种类差异很大。W1组乌鳢肠道细菌的优势类群是邻单胞菌属(Plesiomonas)、狭义梭菌属(Clostridium sensu stricto)、鲸蜡菌属(Cetobacterium);W2组乌鳢肠道细菌的优势类群是狭义梭菌属(Clostridium sensu stricto)、邻单胞菌属(Plesiomonas)、Spartobacteria、甲基副球菌属(Methyloparacoccus)、杆菌属(Alsobacter)、Saccharibacteria、分支杆菌属(Mycobacterium)。W2组乌鳢肠道细菌香农指数高于W1组乌鳢肠道细菌,表明前者肠道细菌多样性大于后者。本实验揭示了畜禽内脏饲喂乌鳢肠道细菌的群落种类多样性、OTU丰富度和物种总数均高于冰鲜鱼饲喂乌鳢;W1组肠道有益菌OTU丰富度大于W2组,且W2组还存在一些潜在致病菌。本研究为乌鳢的健康养殖和后续肠道微生物资源的挖掘提供了理论依据。  相似文献   

福建省复杂地形下旬平均气温分布式模拟     

李威鹏  邱新法  曾燕  施国萍  徐金勤  王丹丹 《热带地理》2019,39(2):229-236

为建立高时空分辨率的福建省复杂地形下气温栅格数据集，利用福建省及其周边33个常规气象站观测资料，基于数字高程模型（DEM）数据，综合考虑海拔、太阳总辐射、地表长波有效辐射对旬平均气温的影响，模拟了福建省复杂地形下旬均温的空间分布。结果表明：1）常规站验证结果显示：各旬气温绝对误差平均值（MAE）最小为0.46℃，最大为2.3℃，全年平均为0.87℃；加密站验证结果显示，MAE最大为2.3℃，最小0.5℃，全年平均为0.96℃。2）模拟结果能反映旬均温的宏观分布规律与局地细节特征。宏观范围内，旬均温受纬度影响较大，由北至南气温逐渐升高，沿海地区旬均温整体高于内陆，山区旬均温明显较低；局地范围内，各坡向上气温差异显著，海拔越高、坡度越大，差异越明显；地形因子对旬平均温的影响具有季节差异，具体表现为冬季时地形因子对旬均温的影响最大，秋季次之，春夏季节中地形因子对旬均温的影响最弱。  相似文献   

近20年来长江经济带生态环境变化          下载免费PDF全文

周权平  张澎彬  薛腾飞  姜月华  郭琳  杨冉冉 《中国地质》2021,48(4):1127-1141

长江经济带发展战略实施以来,长江上、中、下游地区经济快速发展,与此同时,生态系统不可避免受到扰动,长江经济带生态环境状况面临压力,亟需开展长江经济带生态环境质量评价。本文选取2001年和2020年MODIS和Landsat遥感影像计算湿度、绿度、干度、热度4个评价指标,通过主成分分析方法计算遥感生态指数RSEI,对长江经济带11省(市)生态环境现状及变化进行定量分析评价。结果表明长江经济带整体生态环境等级为较优,2001—2020年保持总体向好态势。空间分布上,生态环境优等区域主要分布于武夷山、罗霄山、雪峰山、武陵山、巫山、大巴山、大别山、西双版纳等山地丘陵一带,生态环境较差及差等区域主要分布于大中小城镇等人类聚集地、川西横断山区。时空变化上,生态环境等级下降区域主要集中在长三角太湖周边、江汉平原、洞庭湖周边、安徽西北部、湖南南部及四川盆地等人类聚集地周边,生态环境等级上升区域主要集中在四川盆地大部、重庆山区及三峡库区、贵州大部、云南东南部、安徽中部及北部、江苏北部等地区。  相似文献   

数字倾斜仪资料质量跟踪与映震效能研究     

方燕勋  卞根发  叶建刚  崔翔  钟伟 《地震地磁观测与研究》2011,32(6):138-142

通过跟踪分析湖州地震台数字化水管倾斜仪和垂直摆倾斜仪连续2年的资料质量,发现水管倾斜仪观测精度高于垂直摆倾斜仪,并且资料稳定性逐年提高。从映震效能看,水管倾斜仪同震响应幅度远大于垂直摆,水管倾斜仪的自振周期远大于垂直摆倾斜仪而接近远震面波的周期,形成谐振,但两者的同震响应时间相当。  相似文献   

湖州市用电需求特性及其与气象条件的关系     

盛琼  朱晓东  骆丽楠  顾泽 《南京气象学院学报》2011,34(1):122-127

通过计算日用电量气象变化率lml、日最大用电负荷气象变化率lmh,分析了湖州市2006—2008年用电量及最大用电负荷的变化特征及其与气象要素的关系,着重研究了平均气温、最高气温、最低气温对用电量及最大用电负荷的影响,建立了日最大用电负荷、日用电量的预测模型。结果表明：用电量及最大用电负荷表现出年周期变化,且稳步递增,但月差异明显;不同月份不同气象因子对用电量及最大用电负荷的影响各有不同,lmh、lml与气象因子相关性显著的月份集中在6—10月;在不同温度范围,气温对用电量及最大用电负荷的影响程度也不同,随着气温变化,用电量和最大用电负荷的变化率最大可达20%;在7月、8月,气温升高1℃时,lmh、lml的变化最大,可达2%～5%。  相似文献   

一例异育银鲫(Carassius auratus gibelio)暴发性出血病病原分析     

袁雪梅  潘晓艺  郝贵杰  刘莉  吕孙建  蔺凌云  沈锦玉 《海洋与湖沼》2019,50(4):913-920

2016年6月,江苏某异育银鲫(Carassius auratus gibelio)养殖场暴发一种传染性急性出血病,造成养殖银鲫大量死亡。为分析此次疾病病因及流行规律,本研究从发病养殖场采集患出血病的异育银鲫,从细菌、病毒及寄生虫三个方面对病原进行了分析。采用病原菌分离、组织病理学观察、超薄切片电镜观察、病毒核酸分析、回感实验等对病原进行鉴定。结果显示从发病鲫鱼体内分离到病毒一株,未发现寄生虫及细菌感染。经测序及序列分析,该病毒为鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus2,CyHV-2)病毒,组织病理学观察结果显示患病鱼的鳃和肾脏有明显病变,电镜下可观察到病鱼脾脏组织有带囊膜的球形病毒,囊膜直径约为170—200nm,病毒衣壳直径约为110—120nm,核心直径约为60nm,用组织匀浆感染鲫鱼囊胚细胞系(CGB)可稳定地观察到典型的细胞病变,用患病鱼组织匀浆液人工感染异育银鲫的死亡率高达100%,荧光定量PCR检测到该病毒可感染多器官,其中以脾脏中病毒含量最高,其次是脑,肝脏中最少。本研究可为CyHV-2的诊断防控及疫苗研制提供资料。  相似文献   

A Comprehensive Observational Analysis for the Effects of Gas Cannons on Clouds and Precipitation     

吴彬  王丹丹  李艳芳  尹浩  杜爽  黎朋红 《热带气象学报(英文版)》2022,28(2):237-251

To analyze the effects of gas cannons on clouds and precipitation, multisource observational data, including those from National Centers for Environmental Prediction (NCEP) reanalysis, Hangzhou and Huzhou new-generation weather radars, laser disdrometer, ground-based automatic weather station, wind profiler radar, and Lin''an C-band dual polarization radar, were adopted in this study. Based on the variational dual-Doppler wind retrieval method and the polarimetric variables obtained by the dual-polarization radar, we analyzed the microphysical processes and the variations in the macro - and microphysical quantities in clouds from the perspective of the synoptic background before precipitation enhancement, the polarization echo characteristics before, during and after enhancement, and the evolution of the fine three-dimensional kinematic structure and the microphysical structure. The results show that the precipitation enhancement operation promoted the development of radar echoes and prolonged their duration, and both the horizontal and vertical wind speeds increased. The dual-polarization radar echo showed that the diameter of the precipitation particles increased, and the concentration of raindrops increased after precipitation enhancement. The raindrops were lifted to a height corresponding to 0 to -20 ℃ due to vertical updrafts. Based on the disdrometer data during precipitation enhancement, the concentration of small raindrops (lgNw) showed a significant increase, and the mass-weighted diameter Dm value decreased, indicating that the precipitation enhancement operation played a certain“lubricating”effect. After the precipitation enhancement, the concentration of raindrops did not change much compared with that during the enhancement process, while the Dm increased, corresponding to an increase in rain intensity. The results suggest the positive effect of gas cannons on precipitation enhancement.  相似文献   

大应变静力触探数值模拟及锥形因子影响因素分析   总被引：1，自引：1，他引：0       下载免费PDF全文

肖先波  李波  王婷  邱敏 《水文地质工程地质》2018,(2):70-76

以模拟圆锥在不排水黏土中的静力贯入和分析其影响因素为目标,假设土体为均质弹性—完全塑性材料且服从Mises屈服准则,采用任意拉格朗日—欧拉(ALE)网格划分技术确保锥尖土体在大应变条件下的网格质量,进行大应变有限元数值模拟,并分析了稳定状态下土体刚度指数、原位应力状态和锥尖粗糙程度对塑性区半径与锥形因子的影响,获得了锥形因子表达式。模拟结果表明:塑性区随着刚度指数的增大而增大,锥尖周围塑性区的径向扩张处于柱形孔扩张和球形孔扩张之间,更接近于球形孔扩张;锥形因子随土体刚度指数、锥尖粗糙程度的增大而增大,随土体原位应力状态参数的增大而减小;得到的锥形因子表达式可以量化土体刚度指数、原位应力状态和锥尖粗糙程度的影响,具有较高的精确度。  相似文献   

Singularly perturbed solution of coupled model in atmosphere-ocean for global climate   总被引：1，自引：1，他引：0  

Jiaqi Mo  Wantao Lin  Hui Wang 《中国地理科学(英文版)》2008,18(2):193-196

A box model of the interhemispheric thermohaline circulation （THC） in atmosphere-ocean for global climate is considered. By using the multi-scales method, the asymptotic solution of a simplified weakly nonlinear model is discussed. Firstly, by introducing first scale, the zeroth order approximate solution of the model is obtained. Secondly, by using the multi-scales, the first order approximate equation of the model is found. Finally, second order approximate equation is formed to eliminate the secular terms, and a uniformly valid asymptotic expansion of solution is decided. The multi-scales solving method is an analytic method which can be used to analyze operation sequentially. And then we can also study the diversified qualitative and quantitative behaviors for corresponding physical quantities. This paper aims at providing a valid method for solving a box model of the nonlinear equation.  相似文献   

Modelling the interaction of bentonite with hyperalkaline fluids     

《Applied Geochemistry》2002,17(3):207-223

Many designs for geological disposal facilities for radioactive and toxic wastes envisage the use of cement together with bentonite clay as engineered barriers. However, there are concerns that the mineralogical composition of the bentonite will not be stable under the hyperalkaline pore fluid conditions (pH > 12) typical of cement and its properties will degrade over long time periods. The possible extent of reaction between bentonite and cement pore fluids was simulated using the reaction-transport model, PRECIP. Key minerals in the bentonite (Na-montmorillonite, analcite, chalcedony, quartz, calcite) were allowed to dissolve and precipitate using kinetic (time-dependent) reaction mechanisms. Simulations were carried out with different model variants investigating the effects of: temperature (25 and 70 °C); cement pore fluid composition; dissolution mechanism of montmorillonite; rates of growth of product minerals; solubilities of product minerals; and aqueous speciation of Si at high pH. Simulations were run for a maximum of 3.2 ka. The results of all simulations showed complex fronts of mineral dissolution and growth, driven by the relative rates of these processes for different minerals. Calcium silicate hydrate (CSH) minerals formed closest to the cement-bentonite boundary, whereas zeolites and sheet silicates formed further away. Some growth of primary bentonite minerals (analcite, chalcedony, calcite and montmorillonite) was observed under certain conditions. Most alteration was associated with the fluid of highest pH, which showed total removal of primary bentonite minerals up to 60 cm from the contact with cement after ∼1 ka. The maximum porosity increase observed was up to 80–90% over a narrow zone 1–2 cm wide, close to the cement pore fluid- bentonite contact. All simulations (except that with alternative aqueous speciation data for Si) showed total filling of porosity a few cms beyond this interface with the cement, which occurred after a maximum of 3.2 ka. Porosity occlusion was principally a function of the growth of CSH minerals such as tobermorite. There was very little difference in the alteration attained using different model variants, suggesting that bentonite alteration was not sensitive to the changes in parameters under the conditions studied, so that transport of pore fluid through the bentonite governed the amount of alteration predicted. Principal remaining uncertainties associated with the modelling relate to assumptions concerning the evolution of surface areas of minerals with time, and the synergy between changing porosity and fluid flow/diffusion.  相似文献